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  <front>
    <journal-meta>
      <journal-id journal-id-type="publisher-id">research-focus</journal-id>
      <journal-title-group>
        <journal-title>Research Focus International Scientific Journal</journal-title>
        <abbrev-journal-title abbrev-type="publisher">Research Focus</abbrev-journal-title>
      </journal-title-group>
      <issn pub-type="epub" publication-format="electronic">2181-3833</issn>
      <publisher>
        <publisher-name>Ilm-fan va innovatsiyalar akademiyasi</publisher-name>
      </publisher>
    </journal-meta>
    <article-meta>
      <article-id pub-id-type="doi">10.66073/researchfocus.v4i3.1521</article-id>
      <article-id pub-id-type="other" assigning-authority="relibrary.uz">REL-A-012853</article-id>
      <article-categories>
        <subj-group subj-group-type="oak">
          <subject>14.00.00</subject>
        </subj-group>
      </article-categories>
      <title-group>
        <article-title xml:lang="en">STUDY OF THE ROLE OF ANTIMICROBIAL RESIDUE CONTAMINATION IN CHICKEN MEAT</article-title>
        <trans-title-group xml:lang="ru">
          <trans-title>ИЗУЧЕНИЕ РОЛИ ЗАГРЯЗНЕНИЯ ОСТАТКАМИ АНТИМИКРОБНЫХ ПРЕПАРАТОВ В МЯСЕ КУРИЦЫ</trans-title>
        </trans-title-group>
        <trans-title-group xml:lang="uz">
          <trans-title>TOVUQ GO‘SHTIDA ANTIMIKROB QOLDIQLARI BILAN IFLOSLANISH ROLINI O‘RGANISH</trans-title>
        </trans-title-group>
      </title-group>
      <contrib-group content-type="author">
        <contrib contrib-type="author">
          <string-name>Azizov Yu. D.</string-name>
          <aff>Andijan State Medical Institute</aff>
        </contrib>
      </contrib-group>
      <pub-date publication-format="electronic" date-type="pub" iso-8601-date="2025-04-05">
        <day>05</day>
        <month>04</month>
        <year>2025</year>
      </pub-date>
      <pub-date date-type="collection">
        <year>2025</year>
      </pub-date>
      <volume>4</volume>
      <issue>3</issue>
      <fpage>138</fpage>
      <lpage>141</lpage>
      <permissions>
        <license xlink:href="https://creativecommons.org/licenses/by/4.0">
          <ali:license_ref>https://creativecommons.org/licenses/by/4.0</ali:license_ref>
          <license-p>This work is licensed under a Creative Commons Attribution 4.0 International License.</license-p>
        </license>
      </permissions>
      <self-uri xlink:href="https://relibrary.uz/REL-A-012853/"/>
      <abstract xml:lang="en">
        <p>The investigation aimed to assess bacterial contamination in 120 chicken meat samples collected from various retail marketplaces in Uzbekistan. Samples were analyzed microbiologically by isolating them on Agar medium, performing Gram‘s staining and enzymatic tests for confirmation. Antibacterial compounds were tested using the STAF procedure. The suspected Bacillus subtilis colonies exhibited specific characteristics like yellowish-brown color, smooth margins, and white curly growth, further confirmed by sheep blood agar growth and Gram staining. Biochemical tests confirmed Bacillus subtilis through catalase, gelatin hydrolysis, glucose fermentation, citrate utilization, and nitrate reduction tests. Microscopy revealed green spores and red bacterial cells. The study revealed significant antimicrobial drug residue contamination in chicken meat, likely due to antibiotic misuse or insufficient withdrawal periods. The presence of Bacillus subtilis in poultry meat indicates potential quality degradation and risks to human health. The research aims to enhance food safety policies by identifying contamination sources and antibiotic misuse, ultimately improving poultry product safety. This study not only benefits scientific understanding of microbial dynamics in poultry production but also contributes to reducing foodborne illnesses linked to contaminated meat.</p>
      </abstract>
      <trans-abstract xml:lang="ru">
        <p>Исследование было направлено на оценку бактериального загрязнения в 120 образцах куриного мяса, собранных на различных розничных рынках в Узбекистане. Образцы были проанализированы микробиологически путем выделения их на среде агара, проведения окрашивания по Граму и ферментативных тестов для подтверждения. Антибактериальные соединения были протестированы с помощью процедуры STAF. Предполагаемые колонии Bacillus subtilis демонстрировали специфические характеристики, такие как желтовато-коричневый цвет, гладкие края и белый курчавый рост, что дополнительно подтверждается ростом агара в крови овец и окрашиванием по Граму. Биохимические тесты подтвердили Bacillus subtilis с помощью каталазы, гидролиза желатина, ферментации глюкозы, утилизации цитратов и тестов на снижение нитратов. Микроскопия выявила зеленые споры и эритроциты. Исследование выявило значительное загрязнение остатков противомикробных препаратов в курином мясе, вероятно, из-за неправильного использования антибиотиков или недостаточных периодов отмены. Присутствие Bacillus subtilis в мясе птицы указывает на потенциальное ухудшение качества и риск для здоровья человека. Исследование направлено на совершенствование политики безопасности пищевых продуктов путем выявления источников загрязнения и злоупотребления антибиотиками, что в конечном итоге повышает безопасность продуктов птицеводства. Это исследование не только способствует научному пониманию микробной / динамики в птицеводстве, но и способствует снижению случаев пищевого отравления, связанных с зараженным мясом.</p>
      </trans-abstract>
      <trans-abstract xml:lang="uz">
        <p>Tadqiqot O‘zbekistondagi turli chakana savdo bozorlaridan yig‘ilgan 120 ta tovuq go‘shti namunasidagi bakterial ifloslanishni baholashga qaratilgan edi. Namunalar agar muhitida ajratib olish, Gram bo‘yicha bo‘yash va tasdiqlash uchun fermentativ testlar o‘tkazish yo‘li bilan mikrobiologik jihatdan tahlil qilindi. Antibakterial birikmalar STAF usuli yordamida tekshirildi. Taxmin qilingan Bacillus subtilis koloniyalari sarg‘ish-jigarrang rang, silliq chekkalar va oq jingalak o‘sish kabi o‘ziga xos xususiyatlarni namoyon etdi, bu qo‘y qoni agarida o‘sish va Gram bo‘yicha bo‘yash bilan qo‘shimcha tasdiqlandi. Biokimyoviy testlar Bacillus subtilis ni katalaza, jelatin gidrolizi, glyukoza fermentatsiyasi, sitratdan foydalanish va nitratni qaytarish testlari orqali tasdiqladi. Mikroskopiya yashil sporalar va qizil bakterial hujayralarni aniqladi. Tadqiqot tovuq go‘shtida antimikrob dori qoldiqlari bilan sezilarli ifloslanishni aniqladi, bu ehtimol antibiotiklarni noto‘g‘ri qo‘llash yoki yetarli bo‘lmagan chiqarish muddatlari bilan bog‘liq. Parranda go‘shtida Bacillus subtilis ning mavjudligi sifat pasayishi ehtimoli va inson salomatligi uchun xavfdan dalolat beradi. Tadqiqot ifloslanish manbalari va antibiotiklarni suiiste‘mol qilishni aniqlash orqali oziq-ovqat xavfsizligi siyosatini takomillashtirishga qaratilgan bo‘lib, pirovard natijada parranda mahsulotlari xavfsizligini oshiradi. Ushbu tadqiqot parrandachilikdagi mikrob dinamikasini ilmiy tushunishga hissa qo‘shibgina qolmay, ifloslangan go‘sht bilan bog‘liq oziq-ovqat orqali yuqadigan kasalliklarni kamaytirishga ham xizmat qiladi.</p>
      </trans-abstract>
      <kwd-group xml:lang="en">
        <kwd>Bacillus subtilis</kwd>
        <kwd>chicken meat</kwd>
        <kwd>food safety</kwd>
        <kwd>public health</kwd>
      </kwd-group>
      <kwd-group xml:lang="ru">
        <kwd>Bacillus subtilis</kwd>
        <kwd>куриное мясо</kwd>
        <kwd>пищевая безопасность</kwd>
        <kwd>здоровье населения</kwd>
      </kwd-group>
      <kwd-group xml:lang="uz">
        <kwd>Bacillus subtilis</kwd>
        <kwd>tovuq go‘shti</kwd>
        <kwd>oziq-ovqat xavfsizligi</kwd>
        <kwd>aholi salomatligi</kwd>
      </kwd-group>
    </article-meta>
  </front>
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</article>
